A Red Fluorescent Protein-Based Probe for Detection of Intracellular Reactive Sulfane Sulfur
نویسندگان
چکیده
منابع مشابه
Development of a reversible fluorescent probe for reactive sulfur species, sulfane sulfur, and its biological application.
We report a reversible off/on fluorescent probe for monitoring concentration changes of sulfane sulfur by utilizing the unique ability of sulfane sulfur to bind reversibly to other sulfur atoms and the intramolecular spirocyclization reaction of xanthene dyes. It reversibly visualized sulfane sulfur in living A549 cells and primary-cultured hippocampal astrocytes.
متن کاملA red-emitting ratiometric fluorescent probe based on a benzophosphole P-oxide scaffold for the detection of intracellular sodium ions.
We disclose the development of a ratiometric fluorescent probe based on a benzophosphole P-oxide and its application for the detection of intracellular Na(+) ions. Excitation by visible light induced red emission from this probe in water, which was subjected to a hypsochromic shift upon complexation with Na(+). Based on this change, a ratiometric analysis enabled us to visualise changes in the ...
متن کاملConversion of red fluorescent protein into a bright blue probe.
We used a red chromophore formation pathway, in which the anionic red chromophore is formed from the neutral blue intermediate, to suggest a rational design strategy to develop blue fluorescent proteins with a tyrosine-based chromophore. The strategy was applied to red fluorescent proteins of the different genetic backgrounds, such as TagRFP, mCherry, HcRed1, M355NA, and mKeima, which all were ...
متن کاملQuantum Dot-Fluorescent Protein FRET Probe for Monitoring Intracellular pH
used in this study: His6-mOrange, His6-mOrange M163K, His6-mOrange-Arg9, and His6-mCherry-Arg9. The N-terminal histidine tags were used for protein purification via immobilized metal affinity chromatography (IMAC). mOrange and mOrange M163K are proteins with pH sensitivity in a physiologically-relevant pH range. A polyarginine sequence was appended to the C-terminus of mOrange and mCherry to fa...
متن کاملIntracellular imaging with a graphene-based fluorescent probe.
Graphene is an increasingly important nanomaterial that has shown great promise in the area of nanotechnology. In this study, fluorescein-functionalized graphene oxide (GO) is synthesized via a polyethylene glycol (PEG) bridge and its application in intracellular imaging is explored. GO is an oxide form of graphene that provides an ideal platform to prepare graphene-based functional nanomateria...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Antioxidants
سال: 2020
ISSN: 2076-3921
DOI: 10.3390/antiox9100985